A human prostatic growth factor (hPGF): partial purification and characterization |
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| Authors: | N Nishi Y Matuo Y Muguruma Y Yoshitake K Nishikawa F Wada |
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| Institution: | 1. Department of Endocrinology, Kagawa Medical School, 1750, Miki-cho, Kita-gun, Kagawa 761-07, Japan;2. Department of Biochemistry, Kanazawa Medical University, 1-1, Uchinada-cho, Kahoku-gun, Ishikawa 920-02, Japan;1. Department of Civil Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode 638401, TamilNadu, India;2. M.E Structural Engineering, Bannari Amman Institute of Technology, Sathyamangalam, Erode 638401, TamilNadu, India;1. Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan;2. Division of Oral and Maxillofacial Surgery, Department of Health Science, Course for Oral science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan;3. Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King''s College London, Guy''s Hospital, London Bridge, London, UK;4. Research Center for Advanced Oral Science, Department of Oral Life Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan;5. Faculty of Dental Medicine, University of Airlangga, Surabaya, Indonesia |
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| Abstract: | A growth factor capable of stimulating DNA synthesis of BALB/3T3 cells was purified about 1,000-fold from the cytosol of human benign hypertrophic prostates by heparin-Sepharose chromatography; the growth factor bound to the column in the presence of 0.5 M NaCl was eluted with 1.5-1.7 M NaCl. Its molecular weight and isoelectric point were estimated to be 11,000-13,000 and 10.5, respectively. It was sensitive to heat- and acid-treatments but resistant to disulfide-reducing agent. The final preparation was able to stimulate DNA synthesis at 10 ng/ml. The degree of stimulation was dependent on serum concentration in the assay system; the degree of maximum stimulation increased about 5 times as serum concentration increased from 0.2 to 2%. |
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