| 扁玉螺体表和消化系统甲硫氨酸脑啡肽免疫组织化学定位 |
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| 引用本文： | 李华,孙虎山,王宜艳,李霞.扁玉螺体表和消化系统甲硫氨酸脑啡肽免疫组织化学定位[J].动物学杂志,2008,43(4):72-76. |
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| 作者姓名： | 李华 孙虎山 王宜艳 李霞 |
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| 作者单位： | 鲁东大学生命科学学院,烟台,264025 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 采用免疫组织化学strept avidin-biotin complex(S-ABC)法对甲硫氨酸脑啡肽(methionine-enkephalin,M-ENK)在扁玉螺(Neverita didyma)体表、消化系统各器官中的分布进行了研究.结果表明,扁玉螺的足上皮细胞、外套膜的内外上皮细胞以及食道、胃、肠道的黏膜上皮细胞均呈M-ENK阳性反应,且消化道中的阳性反应多集中于上皮细胞游离端;在食道腺的腺上皮中也有少量阳性细胞分布;肝是M-ENK阳性细胞分布较多的器官,主要分布在肝小叶中腺细胞边缘游离端.M-ENK在扁玉螺体表和消化系统各器官均有分布,且分布密度有所不同,可能与各部位的功能有关.
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| 关 键 词： | 甲硫氨酸脑啡肽 免疫组织化学 消化系统 体表 扁玉螺 |
| 收稿时间： | 2008/1/10 0:00:00 |
| 修稿时间： | 2008/4/23 0:00:00 |
Immunohistochemical Localization of Methionine-enkephalin in the Body Surface and Digestive System of Neverita didyma |
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| LI Hu,SUN Hu-Shan,WANG Yi-Yan and LI Xia.Immunohistochemical Localization of Methionine-enkephalin in the Body Surface and Digestive System of Neverita didyma[J].Chinese Journal of Zoology,2008,43(4):72-76. |
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| Authors: | LI Hu SUN Hu-Shan WANG Yi-Yan and LI Xia |
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| Institution: | LI Hua SUN Hu-Shan WANG Yi-Yan LI Xia(College of Life Science,Ludong University,Yantai 264025,China) |
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| Abstract: | The methionine-enkephalin(M-ENK)in the body surface and digestive system of Neverita didyma was analyzed by the immunohistochemical S-ABC(strept avidin-biotin complex) staining.The results showed that M-ENK positive cells existed widely in the epithelium of foot,inner and outer epithelia of mantle,mucous membrane epithelium of oesophagus,stomach and intestine.Besides,the positive immuno-reaction was centralized in the dissociative sides of alimentary canal epithelium.A small quantity of positive cells was o... |
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| Keywords: | Methionine-enkephalin Immunohistochemistry Digestive system Body surface Neverita didyma |
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