Effects of ginsenoside on pacemaker potentials of cultured interstitial cells of Cajal clusters from the small intestine of mice |
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Authors: | Seungheon Han Jung Soo Kim Bo Kyoung Jung Song Ee Han Joo Hyun Nam Young Kyu Kwon Seung-Yeol Nah Byung Joo Kim |
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Institution: | Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan 626-870, Korea. |
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Abstract: | Ginsenoside, one of the active ingredients of Panax ginseng, has a variety of physiological and pharmacological actions in
various organs. However, little is known about the effects of ginsenosides on gastrointestinal (GI) motility. We studied the
modulation of pacemaker potentials by ginsenoside in the interstitial cells of Cajal (ICCs) using the whole-cell patch clamp
technique in the current clamp mode. Among ginsenosides, we investigated the effects of ginsenoside Rb1, Rg3 and Rf. While
externally applied Rb1 and Rg3 had no effects on pacemaker potentials, Rf caused membrane depolarization. The application
of flufenamic acid or niflumic acid abolished the generation of pacemaker potentials and inhibited the Rf-induced membrane
depolarization. Membrane depolarization induced by Rf was not inhibited by intracellular application of guanosine 5′-β-thio]diphosphate
trilithium salt. Pretreatment with a Ca2+-free solution, thapsigargin, a Ca2+-ATPase inhibitor of the endoplasmic reticulum, U-73122, a phospholipase C inhibitor, or 2-APB, an IP3 receptor inhibitor,
abolished the generation of pacemaker potentials and suppressed Rfinduced actions. However, treatment with chelerythrine and
calphostin C, protein kinase C inhibitors, did not block Rf-induced effects on pacemaker potentials. These results suggest
that ginsenoside Rf modulates the pacemaker activities of ICCs and therby regulates intestinal motility. |
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Keywords: | gastrointestinal (GI) motility ginsenoside interstitial cells of Cajal (ICCs) |
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