|
|||||
|
|
Structure of the Bordetella trematum LPS O-chain subunit |
|
| Authors: | Vinogradov Evgeny Caroff Martine |
| Institution: | Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6. |
| Abstract: | Analysis of the O-chain subunit of the lipopolysaccharide (LPS, endotoxin) isolated from Bordetella trematum, a recently identified human pathogen, was undertaken. The polysaccharide (PS) moiety was shown to contain only two O-chain subunits, which differed in the anomeric bond of their first sugar. A trisaccharide fragment resulting from the cleavage of a FucNAc glycosidic bond was isolated after treatment of the PS with anhydrous HF. Nitrous deamination of the LPS led to the release of the following heptasaccharide corresponding to two trisaccharide subunits linked to an anhydromannitol residue. beta-ManNAc3NAmA-(1-4)-beta-ManNAc3NAmA-(1-3)-alpha-FucNAc-(1-4)-beta-ManNAc3NAmA-(1-4)-beta-ManNAc3NAmA-(1-3)-beta-FucNAc-(1-6)-2,5-anhManol. |
| Keywords: | COSY correlation spectroscopy TOCSY total correlation spectroscopy NOESY nuclear overhauser enhancement spectroscopy HMQC heteronuclear multiple quantum coherence HMBC heteronuclear multiple bond connectivity HSQC heteronuclear single quantum correlation ROESY rotational nuclear overhauser effect spectroscopy LPS lipopolysaccharide MALDI matrix-assisted laser desorption/ionization mass spectrometry FucNAc 2-acetamido-2 6-deoxy-galactose GlcN glucosamine ManNAc3NAcA 2 3-diacetamido-2 3-dideoxy-mannuronic acid ManNAc3NAmA 2-acetamido-3-acetamidino-2 3-dideoxy-mannuronic acid |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |