STUDIES ON THE BIOSYNTHESIS OF COLLAGEN : II. THE CONVERSION OF14C-L-PROLINE TO14C-HYDROXYPROLINE BY FOWL OSTEOBLASTS IN TISSUE CULTURE |
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| Authors: | R. H. Smith Sylvia Fitton Jackson |
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| Affiliation: | From Medical Research Council, Biophysics Research Unit, Wheatstone Laboratory, King''s College, London |
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| Abstract: | 1. Fowl osteoblasts grown in bulk tissue cultures in the presence of 14C-(L)-proline incorporated this amino acid into peptide linkage. A significant amount of the incorporated radioactivity was found in the hydroxyproline, glutamic acid, and aspartic acid fractions of the cultures. 2. The rate of formation of protein-bound 14C-hydroxyproline from 14C-(L)-proline was maximal in cultures grown for 15 hours and fell exponentially with the increasing age of the cultures. 3. 14C-(L)-glutamic acid was incorporated by the osteoblast cultures, but no significant amount was converted to hydroxyproline. |
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