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Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing
Authors:James K. Nuñez  Jin Chen  Greg C. Pommier  J. Zachery Cogan  Joseph M. Replogle  Carmen Adriaens  Gokul N. Ramadoss  Quanming Shi  King L. Hung  Avi J. Samelson  Angela N. Pogson  James Y.S. Kim  Amanda Chung  Manuel D. Leonetti  Howard Y. Chang  Martin Kampmann  Bradley E. Bernstein  Volker Hovestadt  Jonathan S. Weissman
Affiliation:1. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA;2. Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA;3. Department of Urology, University of California, San Francisco, CA 94158, USA;4. Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA 94158, USA;5. Tetrad Graduate Program, University of California, San Francisco, CA 94158, USA;6. Medical Scientist Training Program, University of California, San Francisco, CA 94158, USA;7. Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142, USA;8. Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA;9. Broad Institute of MIT and Harvard, Cambridge, MA 02139, USA;10. Center for Cancer Research, Massachusetts General Hospital, Boston, MA 02129, USA;11. Institute for Neurodegenerative Diseases, University of California, San Francisco, CA 94158;12. Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA;13. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA;14. Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA;15. Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA;16. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA;17. Division of Hematology/Oncology, Boston Children’s Hospital, Boston, MA 02215, USA
Abstract:
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  • Keywords:CRISPR  epigenetics  DNA methylation  cell therapy  dCas9
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