Detection of Bioactive Exometabolites Produced by the Filamentous Marine Cyanobacterium Geitlerinema sp. |
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Authors: | Nelson H Caicedo Jolanta Kumirska Jennifer Neumann Stefan Stolte Jorg Th?ming |
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Institution: | UFT-Centre for Environmental Research and Sustainable Technology, University of Bremen, Leobener Stra?e UFT, 28359, Bremen, Germany. uacocjunior@googlemail.com |
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Abstract: | Marine cyanobacteria are noted for their ability to excrete metabolites with biotic properties. This paper focuses on such exometabolites obtained from the culture of the marine filamentous cyanobacterium Geitlerinema sp. strain, their purification and subsequent analyses. By this means the recoveries of the active compounds, a prerequisite for properly determining their concentration, are quantified here for the first time. We demonstrate a new procedure using Amberlite XAD-1180 resin in combination with the eluent isopropanol for extraction of the culture media and gas chromatography as simplified chemical analysis. This procedure reduced necessary bacteria cultivation time (from 150 to 21?days) at low volumes of culture media (300?mL) required for identification of two selected bioactive compounds: 4,4'-dihydroxybiphenyl and harmane. |
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