| 高效液相色谱法测定川射干鸢尾苷元磺酸钠及其制剂泰克吉宁注射液含量和有关物质 |
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| 引用本文： | 袁崇均,王笳,陈帅,罗森,周天玉,徐学民. 高效液相色谱法测定川射干鸢尾苷元磺酸钠及其制剂泰克吉宁注射液含量和有关物质[J]. 天然产物研究与开发, 2010, 22(1) |
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| 作者姓名： | 袁崇均 王笳 陈帅 罗森 周天玉 徐学民 |
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| 作者单位： | 四川省中医药科学院,成都,610041 |
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| 基金项目： | 国家重大科技专项"创新药物和中药现代化"项目 |
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| 摘 要： | 建立高效液相色谱法测定鸢尾苷元磺酸钠(4′,5,7-三羟基-6-甲氧基异黄酮-5′-磺酸钠)及其制剂泰克吉宁注射液的含量及有关物质。采用C18色谱柱(150×4.6mm,5μm),流动相为甲醇-5%醋酸水(80:20),流速为1.0mL/min,检测波长为263nm。鸢尾苷元磺酸钠在11～100μg/mL范围内线性关系良好,回归方程Y=43.3609X-1.5973(r=0.99998),平均回收率为99.77%(n=9)。最低检测限为0.052μg/mL,与有关物质分离良好。本法快速、简便、准确,专属性强。
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| 关 键 词： | 鸢尾苷元磺酸钠 泰克吉宁注射液 含量测定 有关物质 高效液相色谱法 |
HPLC Determination of the Content and the Related Substances of Tectorigenin Sodium Sulfonite and Its Preparation Taike Jining Injection |
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| YUAN Chong-jun,WANG Jia,CHEN Shuai,LUO Sen,ZHOU Tian-yu,XU Xue-ming. HPLC Determination of the Content and the Related Substances of Tectorigenin Sodium Sulfonite and Its Preparation Taike Jining Injection[J]. Natural Product Research and Development, 2010, 22(1) |
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| Authors: | YUAN Chong-jun WANG Jia CHEN Shuai LUO Sen ZHOU Tian-yu XU Xue-ming |
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| Affiliation: | YUAN Chong-jun,WANG Jia,CHEN Shuai,LUO Sen,ZHOU Tian-yu,XU Xue-mingSichuan Academy of Chinese Medicine Sciences,Sichuan Chengdu 610041 |
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| Abstract: | To establish an HPLC method to determine the content and the related substances of teetorigenin sodium sulfo-nite (4' ,5,7-trihydroxy-6-methoxy-7-hydroxymethylisotlavone-5'-sulphoric acid sodium) and its preparation-Taikejining Injection.The HPLC system consisted of C18 column(150 × 4.6 mm,5 μm) ,methanol-5% acetic acid solution(80:20) as mobile phase with UV detection at 263 nm,flow rate: 1.0 mL/min, The linear range was 11-100 μg/mL, r =0.99998 ,the average recovery was 99.77% (n =9).The detection limit was 0.052 μg/mL.The related substances were separated well from tectorigenin sodium sulfonite.This method is rapid, simple, specific and accurate. |
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| Keywords: | HPLC tectorigenin sodium sulfonite Taike Jining Injection determination related substance HPLC |
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