Interleukin-1beta stimulates cyclic GMP efflux in brain astrocytes. |
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Authors: | C E Pedraza M A Baltrons A García |
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Institution: | Instituto de Biotecnología y Biomedicina 'V. Villar Palasí' and Departamento de Bioquímica y Biología Molecular, Universidad Autónoma de Barcelona, Bellaterra (Barcelona), Spain. |
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Abstract: | In rat brain astroglia-enriched cultures long-term treatment with interleukin-1beta induces NO release and stimulation of soluble guanylyl cyclase. The cGMP formed is recovered in the extracellular medium but not in the cell monolayer. The interleukin-1beta effect is mediated by type I receptor and potentiated by interferon-gamma. In cells treated with bacterial endotoxin a larger NO-dependent cGMP accumulation occurs only intracellularly, however a significant cGMP egression is observed when cells are co-treated with interleukin-1beta. The non-selective anion transport inhibitors probenecid and verapamil block cGMP efflux, indicating that interleukin-1beta stimulates a cGMP transporter. |
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