Sertoli细胞饲养层对小鼠精原干细胞增殖的影响

摘要 目的 以小鼠睾丸支持细胞(Sertoli)为饲养层，小鼠胚胎成纤维细胞(STO) 饲养层做对照，研究它对小鼠精原干细胞增殖的影响。方法 用无血清StemPro-34 SFM培养基培养2～5日龄小鼠精原干细胞，分别用相差显微镜观察，免疫组化法研究Sertoli饲养层对精原干细胞生物学行为的影响。结果 发现精原干细胞在Sertoli及STO两种饲养层上的一周内的生物学行为非常相似，但培养1周后，Sertoli细胞作饲养层的培养体系中保留的精原干细胞要比对照组明显增多，约有30%的精原干细胞能存活下来并能维持存活到60d以上。结论 Sertoli细胞作饲养层明显促进精原干细胞的更新增殖。

The effect of Sertoli cells feeders on mouse Spermatogonial stem cells in vitro

Abstract：Objective Spermatogonial stem cells （SSCs）dissociated from 2～5 days postpartum mice were cultured on Sertoli cells feeders，to study the effect of Sertoli cells feeders on culture of mouse spermatogonial stem cells．Methods Specific markers CD9 of mouse SSCs cultured serum-free StemPro-34 SFM culture medium were identified by immunohistochemical assay. Result During the first week of culture，on Sertoli cells feeders or STO feeders，the biologica1 behaviors of spermatogonial stem cells showed no obvious difference. After a week of culture，compared with control ,there were more number of spermatogonial stem cells remained when they were cultured for 60 days. These cells were expressed CD9 positive. In conclusion Sertoli cells can be used as feeders not only to promote survival but also renewal of spermatogonial stem cells．