Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma,grown serum-free |
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| Authors: | Zwe-Ling Kong Misao Miwa Hiroki Murakami Kazuki Shinohara |
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| Affiliation: | (1) Institute of Food Chemistry, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Hakozaki, Higashi-ku, 812 Kukuoka, Japan;(2) Food Additive Laboratory, Food Protection and Preservation Division, National Food Research Institute, The Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, 305 Ibaraki, Japan;(3) Laboratory of Cellular Regulation Technology, Graduate School of Genetic Resources Technology, Kyushu University, Hakozaki, Higashi-ku, 812 Fukuoka, Japan |
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| Abstract: | Summary A human macrophagelike cell line which grows in serum-free medium was established from a histiocytic lymphoma cell line, U-937. U-937 cells failed to differentiate into macrophagelike cells in serum-free medium plus 12-O-tetradecanoyl phorbol 13-acetate (TPA). Fibronectin and albumin in serum were necessary for differentiation of U-937 ceds into macrophagelike cells in enriched RDF medium supplemented with insulin, transferrin, ethanolamine, selenite, egg yolk lipoprotein (eRDF-ITESL medium). The established cell line exhibited several characteristic properties of macrophage such as nitroblue tetrazolium reduction, phagocytic and α-naphthylbutyrate-esterase activities, and tumor necrosis factor and interleukin 1 production. At present the cells have been continuously maintained in eRDF-ITESL medium through over 150 passages. |
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| Keywords: | U-937 U-937-derived macrophagelike cell serum-free culture eRDF-ITESL medium |
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