The fluorescence localization of mouse satellite DNA in interphase nuclei |
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| Authors: | John E. Mayfield John R. Ellison |
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| Affiliation: | (1) Department of Biological Sciences, Mellon Institute of Science, Carnegie-Mellon University, 4400 Fifth Avenue, 15213 Pittsburgh, Pennsylvania, USA |
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| Abstract: | Nuclei from various mouse tissues exhibit a pattern of fluorescence characteristic of the cell type when stained with the fluorescent compound Hoechst 33258. When such preparations are hybridized in situ with 3H-RNA complementary to the A-T rich satellite of mouse, it is clearly seen that only the fluorescent regions of the nuclei contain the satellite DNA. Thus Hoechst 33258 allows the precise localization of satellite DNA at all stages of the mouse cell cycle. |
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