Efficient procedure of preparation and properties of long uniform G4-DNA nanowires |
| |
Authors: | Borovok Natalia Molotsky Tatiana Ghabboun Jamal Porath Danny Kotlyar Alexander |
| |
Affiliation: | Department of Biochemistry, George S. Wise Faculty of Life Sciences, Tel Aviv University, Aviv 69978, Israel. |
| |
Abstract: | Here we describe a novel and efficient procedure for preparation of long uniform G4-DNA wires. The procedure includes (i) enzymatic synthesis of double-stranded DNA molecules consisting of long (up to 10,000 bases), continuous G strands and chains of complementary (dC)20-oligonucleotides, poly(dG)-n(dC)20; (ii) size exclusion HPLC separation of the G strands from the (dC)20 oligonucleotides in 0.1M NaOH; and (iii) folding of the purified G strands into G4-DNA structures by lowering the pH to 7.0. We show by atomic force microscopy (AFM) that the preparation procedure yielded G4-DNA wires with a uniform morphology and a narrow length distribution. The correlation between the total amount of nucleotides in the G strands and the contour length of the G4-DNA molecules estimated by AFM suggests monomolecular folding of the G strands into quadruplex structures. The folding takes place either in the presence or in the absence of stabilizing ions (K+ or Na+). The addition of these cations leads to a dramatic change in the circular dichroism spectrum of the G4-DNA. |
| |
Keywords: | G4-DNA nanowires Enzymatic synthesis Folding of poly(dG) strands AFM CD spectroscopy |
本文献已被 ScienceDirect PubMed 等数据库收录! |