Sensitive measurement of polyethylene glycol-modified proteins |
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| Authors: | Tsai N M Cheng T L Roffler S R |
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| Affiliation: | Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. |
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| Abstract: | An IgM monoclonal antibody (AGP3) against polyethylene glycol (PEG) was used to assay PEG-modified proteins by ELISA. PEG-modified beta-glucuronidase could be measured at concentrations as low as 15 ng/mL, corresponding to 750 pg (1.8 fmol) of conjugate. This ELISA should be generally applicable to all PEG-modified proteins because AGP3 binds the backbone of the PEG chain independent of the linker used for PEG attachment. |
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