Quantitation of Bacillus clausii in biological samples by real-time polymerase chain reaction |
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| Authors: | Perotti Mario Mancini Nicasio Cavallero Annalisa Carletti Silvia Canducci Filippo Burioni Roberto Clementi Massimo |
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| Institution: | Università Vita-Salute San Raffaele and Laboratorio di Microbiologia e Virologia, Diagnostica e Ricerca San Raffaele, Milan, Italy. mario.perotti@hsr.it |
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| Abstract: | A real-time PCR assay targeting the highly specific erm34 sequence of Bacillus clausii DNA was developed and optimized. The quantitative assay showed a sensitivity level of 10(2) CFU/microl of sample. The method may represent a useful tool for monitoring the role of B. clausii as probiotic in vivo. |
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