Genomic DNA analysis from mouse toe lysates

The protocol described in this report provides a simple, accurate and efficient assay for detection of transgenes and mutations in large colonies of rodents, using crude lysates prepared from the digit cut from the animals for identification purposes. This can be done as early as 6 days of age, minimizing trauma to the mice and allowing assays to be completed long before weaning. Editor's note: The US Food and Drug Administration (FDA) had amended its regulations on good laboratory practice (GLP) for nonclinical laboratory studies regarding humane procedures for animal identification and expreimentation (Vol. 54 # 75). While discouraging use of toe clipping for animal identification, the FDA charged each facility's Institutional Animal Care and Use Committee (IACUC) with the responsibility for evaluation and final approval. C.A.P.