15-Cis-carotenoids found in the reaction center of a green sulfur bacterium Chlorobium tepidum and in the Photosystem I reaction center of a cyanobacterium Synechococcus vulcanus |
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| Authors: | Bialek-Bylka Grazyna E. Fujii Ritsuko Chen Chun-Hai Oh-oka Hirozo Kamiesu Akihisa Satoh Kazuhiko Koike Hiroyuki Koyama Yasushi |
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| Affiliation: | (1) Faculty of Science, Kwansei Gakuin University, Uegahara, Nishinomiya, 662-8501, Japan (present address: Institute of Physics, Poznan University of Technology, 60-965 Poznan, Poland);(2) Kwansei Gakuin University, Uegahara, Nishinomiya, 662-8501, Japan;(3) Faculty of Science, Kwansei Gakuin University, Uegahara, Nishinomiya, 662-8501, Japan (present address: Department of Chemistry, Jilin University, Changchun, P.R. China);(4) Department of Biology, Faculty of Science, Osaka University, Machikane-yama, Toyonaka, Osaka, 560-0043, Japan;(5) Department of Lifescience, Faculty of Science, Himeji Institute of Technology, Harima Science Garden City, Hyogo, 678-1297, Japan |
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| Abstract: | Carotenoids were extracted, at  4 °C in complete darkness and under nitrogen atmosphere, from the reaction center (RC) of a green-sulfur bacterium and the Photosystem (PS) I RC of a cyanobacterium; each extract was subjected to high-performance liquid chromatography (HPLC) using an apparatus equipped with a two-dimensional diode-array detector in order to spectroscopically identify cis–trans carotenoids while performing HPLC analysis. In the extract from the RC of Chlorobium tepidum, 15-cis and all-trans- -carotenes as well as 13-cis-, 15-cis- and all- trans-chlorobactenes (in the order of elution) were identified, whereas in the extract from the PS I RC of Synechococcus vulcanus, 15-cis-, all-trans- and 9-cis- -carotenes were found. Thus, the universal presence of 15- cis carotenoids in the 'iron sulfur-type' RCs has been shown in addition to the previous cases of the 'quinone-type' RCs. |
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| Keywords: | carotenoids 15-cis configuration |
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