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Light increases the ion and non-electrolyte permeability of rhodopsin-phospholipid vesicles. |
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| Authors: | A Darszon M Montal J Zarco |
| Affiliation: | Departamento de Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México 14, D.F., Mexico |
| Abstract: | Rhodopsin-phospholipid vesicles were formed and loaded with radioactive markers by sonication. Exposure of the vesicles to light increased the permeability to Na+, Cs+, Ca2+, glycerol and glucose, but not to Cl?, sucrose and inulin. These results indicate that light induces the formation of a wide permeability pathway with an apparent cut-off diameter of about 10Å. |
| Keywords: | ROS rod outer segments CTAB cetyltrimethylammonium bromide PB phosphate buffer IB imidazole-HCl buffer DEAE diethylaminoethyl EM electron microscopy difference in absorbance at 500 nm between dark and bleached rhodopsin samples |
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