Regulation of nitrate reduction in a cyanobacterium Phormidium uncinatum: Distinctive modes of ammonium-repression of nitrate and nitrite reductases |
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| Authors: | A Palod VS Chauhan SN Bagchi |
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| Institution: | Department of Biochemistry and Biophysics, University of California, Davis, CA, U.S.A. |
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| Abstract: | Abstract A 5.8 kbp DNA fragment from Clostridium cellulovorans (ATCC 35296) containing endo-β-1,4-glucanase (1,4-β- d -glucan glucanohydrolase, carboxymethylcellulase, CMCase; EC 3.2.1.4) gene, engD was cloned in Escherichia coli . The clone harboring a subcloned 3.8 kb fragment in plasmid, pEQ52V, produced an enzyme that showed both endo-β-1,4-glucanase activity as well as cellobiosidase activity. Zymograms with the engD encoded enzyme with carboxymethyl-cellulose as the substrate indicated that the molecular mass of the active protein was 50 000. |
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| Keywords: | Clostridium cellulovorans Endoglucanase Cellobiosidase Molecular cloning |
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