Platelet-activating factor induces matrix metalloproteinase-9 expression through Ca(2+)- or PI3K-dependent signaling pathway in a human vascular endothelial cell line |
| |
Authors: | Ko Hyun-Mi Kang Jee-Hae Choi Jung-Hwa Park Sung Jun Bai Suk Im Suhn-Young |
| |
Institution: | Department of Biological Sciences, The Institute of Basic Sciences, Chonnam National University, Kwangju 500-757, Republic of Korea. |
| |
Abstract: | Platelet-activating factor (PAF) augments angiogenesis by promoting the synthesis of a variety of angiogenic factors, via the nuclear factor (NF)-kappaB activation. Recently, we reported that PAF upregulates MMP-9 expression in a NF-kappaB-dependent manner. In this study, we investigated the signaling pathway involved in PAF-induced MMP-9 expression in ECV304 cells. Our current data indicate that the Ca(2+)- or phosphatidylinositol 3-kinase (PI3K)-dependent signaling pathway is necessary for PAF-induced MMP-9 expression. Furthermore, PAF-induced NF-kappaB activation was blocked by selective inhibitors of Ca(2+), PI3K, or extracellular signal-regulated kinase (ERK). Our results suggest that PAF-induced MMP-9 expression, in a NF-kappaB-dependent manner, is regulated by Ca(2+), PI3K and ERK signaling pathways. |
| |
Keywords: | MMP matrix metalloproteinase PAF platelet-activating factor PI3K phosphatidylinositol 3-kinase ERK extracellular signal-regulated kinase MAPK mitogen-activating protein kinase |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|