Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity |
| |
Authors: | José Ruiz-Herrera Patricia García-Maceira Luis C Castillo-Barahona Eulogio Valentín Rafael Sentandreu |
| |
Institution: | (1) Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del I.P.N, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto, México;(2) Departamento de Microbiología, Facultad de Farmacia, Universidad de Valencia, Avgda. Vicent Andrés Estelles s/n, Burjassot València, 46100, Spain |
| |
Abstract: | A collection of transposon-mutagenized strains of Yarrowia lipolytica was screened for wall defects by determination of their sensitivity to calcofluor white. A number of strains were hypersensitive,
whereas others were resistant. Different non-allelic mutants displayed increased sensitivity to autolysis and lytic enzymes,
independently of whether they were sensitive or resistant to calcofluor white. A thorough analysis of their cell walls revealed
minor quantitative alterations, and no significant changes in chitin content. Electrophoretic analysis of wall-bound and excreted
proteins proved to be a sensitive method that revealed defects in the cell wall structure of the mutants. Important alterations
in the patterns of the wall proteins extracted by SDS or by enzymatic treatments were noticed for the mutants, as compared
to the parental strain. Mutants released to the growth medium a larger number of protein species than the parental strain,
suggesting impairment in wall assembly of certain polypeptides. Patterns of wall-bound and excreted proteins, as well as alterations
in wall chemical composition were not diagnostic of calcofluor white sensitivity or resistance, but were specific for each
mutant. Our data show that an increase in either sensitivity or resistance of Y. lipolytica to certain levels of calcofluor is equally indicative of alterations in cell wall structure, independent of chitin levels.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | Calcofluor white Lytic enzymes Wall mutants Wall proteins |
本文献已被 PubMed SpringerLink 等数据库收录! |
|