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Culture of droplets containing asparagus cells and protoplasts on polypropylene membrane |
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| Authors: | Chee-Kok Chin Yang Kong Henrik Pedersen |
| Affiliation: | (1) Department of Horticulture and Forestry, Rutgers University, The State University of New Jersey, Blake Hall, P.O. Box 231, 08903 New Brunswick, NJ, USA;(2) Department of Chemical Engineering, Rutgers University, The State University of New Jersey, Blake Hall, P.O. Box 231, 08903 New Brunswick, NJ, USA;(3) China National Rice Research Institute, Hangzhou, People's Republic of China |
| Abstract: | A method of using a buoyant polypropylene membrane floated on liquid medium to culture asparagus cells and protoplasts was examined. Compared with direct culture in lquid medium the method using a polypropylene membrane was found to be superior for small-volume culture of cells at low density as well as for the culture of protoplasts. |
| Keywords: | polypropylene membrane protoplast cell culture |
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