Characterization of bovine seminal plasma by proteomics |
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Authors: | Kelly Van C Kuy Sulee Palmer David J Xu Zhenzhong Davis Stephen R Cooper Garth J |
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Institution: | School of Biological Sciences, University of Auckland, Auckland, New Zealand. |
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Abstract: | Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species. |
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Keywords: | Bovine Proteome analysis Seminal plasma |
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