Analysis of Tween 80 as an esterase/ lipase substrate for lipolytic activity assay |
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| Authors: | Francisco J. Plou Manuel Ferrer Oscar M. Nuero Maria V. Calvo Miguel Alcalde Fuensanta Reyes Antonio Ballesteros |
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| Affiliation: | (1) Instituto de Catalisis, CSIC, Campus Universidad Autonoma, Madrid, Spain;(2) Centro de Investigaciones Biologicas, CSIC, Madrid, Spain |
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| Abstract: | The Tween 80 assay to detect lipolytic activities in agar media was evaluated. A spectrophotometric assay for Tween 80 hydrolysis was established. The specific activities with Tween 80, as well as with some conventional lipase-type and esterase-type substrates, were measured using several lipases and esterases. The activity with Tween 80 was similar to that obtained with p-nitrophenyl butyrate; the enzyme activities with both substrates were between the esterase and lipase categories. © Rapid Science Ltd. 1998 |
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