Special features of immune response to the lethal toxin of Bacillus anthracis |
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Authors: | E V Belova A V Kolesnikov M Yu Zakharova S A Dubiley I A Dyatlov I G Shemyakin |
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Institution: | (1) State Research Center for Applied Microbiology and Biotechnology, Federal Service of Surveillance in the Field of Consumer Rights Protection and Human Welfare, Obolensk, Moscow oblast, 142279, Russia;(2) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia |
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Abstract: | We and other authors have recently shown that the pattern of the immune response to components of anthrax, the Bacillus anthracis lethal toxin, is complex. In addition to the neutralizing antibodies, the antitoxin antibody pool contains antibodies enhancing the toxin lethal action. We mapped the epitopes in the protective antigen that are responsible for the induction of both antibody types. In this study, we obtained new data on the cytotoxicity of the B. anthracis lethal toxin toward the J774 A.1 cell line in the presence of monoclonal antibodies to various domains of the protective antigen and the lethal factor. The role of the Fc fragment of immunoglobulins in enhancing the lethal toxin action was shown. These results may serve as a basis for the development of a new generation vaccine for anthrax. |
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Keywords: | anthrax epitope mapping lethal factor protective antigen neutralizing and enhancing antibodies |
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