Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha |
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Authors: | Kim M D Kang H A Rhee S K Seo J H |
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Institution: | (1) Department of Food Science and Technology and Research Center for New Biomaterials in Agriculture, Seoul National University, Suwon 441-744, Korea, KR;(2) Korea Research Institute of Bioscience and Biotechnology, Taejon 305-600, Korea, KR |
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Abstract: | A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation
characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol
concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration
of 1.7 g l−1 was determined at a dilution rate of 0.18 h−1 with 1.8 mg l−1 h−1 hirudin productivity. Journal of Industrial Microbiology & Biotechnology (2001) 27, 58–61.
Received 21 September 2000/ Accepted in revised form 10 June 2001 |
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Keywords: | : hirudin Hansenula polymorpha methanol oxidase promoter methanol |
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