Kaurenolide biosynthesis in a cell-free system from Cucurbita maxima seeds |
| |
Authors: | Peter Hedden Jan E. Graebe |
| |
Affiliation: | Pflanzenphysiologisches Institut, Untere Karspüle 2, 3400 Göttingen, West Germany |
| |
Abstract: | A new product obtained by incubation of [2-14C ]-mevalonic acid with a cell-free system from Cucurbita maxima endosperm was identified by GC-MS as ent-kaura-6,16-dien-19-oic acid. When this compound was reincubated with the microsomal fraction it was converted to 7β-hydroxykaurenolide and hence to 7β,12α-dihydroxykaurenolide. The dienoic acid was also obtained by incubation of ent-kaurene, ent1-kaurenol, ent-kaurenal and ent-kaurenoic acid, but not ent-7α-hydroxykaurenoic acid, with the microsomal fraction. Thus, in the C. maxima cell-free system, the kaurenolides are formed by a pathway which branches from the GA pathway at ent-kaurenoic acid and proceeds via the dienoic acid. |
| |
Keywords: | Cucurbitaceae biosynthesis cell-free system kaurenolides gibberellins |
本文献已被 ScienceDirect 等数据库收录! |
|