Cloning and characterisation of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase |
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Authors: | Michael A Roberts John L Wray |
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Institution: | (1) Plant Sciences Laboratory, Research Division of Environmental and Evolutionary Biology, School of Biological and Medical Sciences, University of St Andrews, Sir Harold Mitchell Building, KY16 9TH St Andrews, Fife, UK |
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Abstract: | We have cloned an Arabidopsis thaliana cDNA encoding serine acetyltransferase (EC 2.3.1.30) by functional complementation of the Escherichia coli cysE mutant JM15. The cDNA clone Sat-1 conferred serine acetyltransferase activity (with apparent K
m for the two substrates acetyl CoA and L-serine of 0.043 and 3.47 mmol/dm3 respectively) on the cysE mutant. The 1515 bp full-length cDNA encodes a deduced protein of 391 amino acids which includes a putative chloroplastic targeting presequence. Northern analysis revealed a single message of 1.5 kb, while Southern hybridisation suggests a small multigene family of related sequences. |
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Keywords: | Serine acetyltransferase Arabidopsis thaliana YES" target="_blank">gif" alt="lambda" align="BASELINE" BORDER="0">YES functional complementation cysteine biosynthesis |
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