A gas phase chromatography method for determination of low dissolved CO2 concentration and/or CO2 solubility in microbial culture media

CO₂ concentration in aqueous samples was derived from determination of the CO₂ content in the gas phase at equilibrium with the liquid by gas chromatography. This technique was used to measure low dissolved CO₂ concentrations, down to 10^–6 mol/L, and CO₂ solubility in microbial culture media. The method gave results with an accuracy within 10%, and appeared thus as reliable and sensitive. It could be considered as a valuable tool to study CO₂ mass transfer in bio-processes.