TDZ-specific plant regeneration in salad burnet |
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Authors: | Babaoglu M Yorgancilar M |
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Institution: | (1) Faculty of Agriculture, Department of Field Crops, University of Selcuk, 42031, Kampus, Konya, Turkey |
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Abstract: | Various explants of salad burnet (Poterium sanguisorba L.=Sanguisorba minor Scop.) were cultured on semi-solid MS or B5 media containing factorial combinations of various plant growth regulators. Hypocotyl
and petiole explants, after initial callus stage, regenerated prolific adventitious shoots via organogenesis, when placed
on Murashige and Skoog basal medium containing 1–2 μM α-naphthaleneacetic acid and 4-20 μM thidiazuron. Any other growth regulator
combination tested failed to respond. The addition of the biocide, Plant Preservative Mixture, was effective in controlling
contamination and did not impair regeneration. Elongated shoots at 2–4 cm were transferred to rooting medium containing semi-solid
Murashige and Skoog plus 1 μM α-naphthaleneacetic and rooted plants were transferred to the glasshouse. This is the first
report on in vitro plant regeneration within the genusPoterium.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | hypocotyl organogenesis Poterium sanguisorba L PPM thidiazuron |
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