Glucose deprivation induced upregulation of phosphoenolpyruvate carboxykinase modulates virulence in Leishmania donovani |
| |
Authors: | Savita Saini Ayan Kumar Ghosh Ruby Singh Sushmita Das Kumar Abhishek Ajay Kumar Sudha Verma Abhishek Mandal Abul Hasan Sardar Bidyut Purkait Ashish Kumar Kislay Kumar Sinha Pradeep Das |
| |
Affiliation: | 1. Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), Hajipur, Bihar, India;2. Division of Molecular Biology, Rajendra Memorial Research Institute of Medical Sciences (RMRIMS), Indian Council of Medical Research, Patna, Bihar, India;3. Department of Microbiology, All India Institute of Medical Sciences, Patna, Bihar, India |
| |
Abstract: | Various physiological stimuli trigger the conversion of noninfective Leishmania donovani promastigotes to the infective form. Here, we present the first evidence of the effect of glucose starvation, on virulence and survival of these parasites. Glucose starvation resulted in a decrease in metabolically active parasites and their proliferation. However, this was reversed by supplementation of gluconeogenic amino acids. Glucose starvation induced metacyclogenesis and enhanced virulence through protein kinase A regulatory subunit (LdPKAR1) mediated autophagy. Glucose starvation driven oxidative stress upregulated the antioxidant machinery, culminating in increased infectivity and greater parasitic load in primary macrophages. Interestingly, phosphoenolpyruvate carboxykinase (LdPEPCK), a gluconeogenic enzyme, exhibited the highest activity under glucose starvation to regulate growth of L. donovani by alternatively utilising amino acids. Deletion of LdPEPCK (Δpepck) decreased virulent traits and parasitic load in primary macrophages but increased autophagosome formation in the mutant parasites. Furthermore, Δpepck parasites failed to activate the Pentose Phosphate Pathway shunt, abrogating NADPH/NADP+ homoeostasis, conferring increased susceptibility towards oxidants following glucose starvation. In conclusion, this study showed that L. donovani undertakes metabolic rearrangements via gluconeogenesis under glucose starvation for acquiring virulence and its survival in the hostile environment. |
| |
Keywords: | |
|
|