Production and Purification of Antibody to Bovine White Matter Proteolipid Apoprotein |
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| Authors: | Wendy B Macklin Gerald A Schwarting Marjorie B Lees Steven R Cohen |
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| Institution: | Department of Biochemistry, E. K. Shriver Center, Waltham, Massachusetts;Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts;Department of Neurology, Johns Hopkins Medical School, Baltimore, Maryland |
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| Abstract: | Circulating antibody to the bovine white matter proteolipid apoprotein was detected in rabbits 1 month after a single injection of the water-soluble form of the apoprotein. By double immunodiffusion, the antiserum reacted specifically with the delipidated proteolipid apoprotein and the crude proteolipid fraction containing complex lipids; after exposure of the proteolipid apoprotein to sodium dodecyl sulfate (SDS), no reactivity was observed. The antiserum did not react with other myelin components, i.e., basic protein, cerebroside or GM1 ganglioside, nor was there reactivity with non-neural proteolipids. The anti-apoprotein antibody was purified by affinity chromatography. The antibody-antigen interaction is apparently very hydrophobic, since elution of the antibody from the affinity column requires buffer containing 0.5% Triton X-100-4 M-urea. |
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| Keywords: | Antibody Proteolipid Immunodiffusion White matter |
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