Culture condition ofPseudomonas aeruginosa F722 for biosurfactant production |
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Authors: | Kyung-Taek Oh Chang-Min Kang Motoki Kubo Seon-Yong Chung |
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Institution: | (1) Fats, Oils and Animal Coproducts Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA |
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Abstract: | Pseudomonas aeruginosa F722 produces a biosurfactant (BS) during its degradation of carbon and hydrocarbon compounds. The culture conditions for
upgrading the biosurfactant productivity were investigated. The concentration of the biosurfactant produced byP. aeruginosa F722 was 0.78 g/L in C-medium; however, this increased to 1.66 g/L in BS medium, which was experimentally adjusted to optimal
conditions. NaNO2 was found to be most effective for microbial growth, with an O.D600nm of 1.18 for 0.1% NaNO2. Microbial growths, according to the O.D600nm were 2.53, 2.68, 2.89, and 2.87 for glucose, glycerol,n-C10, andn-C22, respectively. Clear zone diameters (cm), indicating biosurfactant activity, were 9.0, 8.8, 5.7, and 8.5 for glucose, glycerol,n-C10, andn-C22, respectively. Microbial growth was not consistent with the biosurfactant activity. The best biosurfactant activity was found
with a C/N ratio of 20. Under optimal culture condition, the average surface tension decreased from 70 to 30 mN/m after 5
days. With aeration of 1.0 vvm, the biosurfactant produced increased to 1.94 g/L (up to 20%) compared to that of 1.66 g/L
with no aeration. With aeration, the velocities of glucose degradation during both the log and stationary growth phases increased
from 0.25 and 0.18 h−1 to 0.33 and 0.29 h−1, respectively, and the time for the culture to arrive at the maximum clear zone diameter became shorter, from 80 down to
60 h with no aeration. |
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