Protein and Ribonucleic Acid Synthesis by Plant Mitochondrial Systems |
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Authors: | BISWENDU B. GOSWAMI SYAMALIMA CHAKRABARTI D. K. DUBE S. C. ROY |
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Affiliation: | Department of Biochemistry, University College of Science, 35 Ballygunge Circular Road, Calcutta 700019, India |
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Abstract: | The incorporation of 14C-labelled phenylalanine into proteins of the mitochondrial systems obtained from 48-h germinating seeds of Vigna sinensis (L.) Savi can be stimulated by polyuridylic acid [poly (U)] and depressed by rifampicin, which is, however, ineffective if poly (U) is allowed to interact with the incorporating system before the antibiotic has access to it. A system consisting of a mitochondrial S-100 fraction and ribosomes from the same source with other cofactors can bring about polymerization of phenylalanine. The incorporation of 14C-labelled uracil into RNA by the plant mitochondria is greatly dependent on the exogenous addition of adenine, guanine, cytosine and also on 5-phosphoribosyl-l-pyrophosphate (5-PRPP). It is greatly suppressed by rifampicin and ethidium bromide. |
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