Lysolecithinase activity in subcellular fractions of rat organs. |
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Authors: | Z Leibovitz-Ben Gershon S Gatt |
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Institution: | Laboratory of Neurochemistry, Department of Biochemistry, Hebrew University - Hadassah Medical School, Jerusalem, Israel |
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Abstract: | Lysolecithinase activity was measured in subcellular fractions of rat liver, kidney, lung and intestine and compared to similar findings in brain. To obtain optimal assay conditions, each fraction was subjected to a kinetic analysis in the absence and presence of albumin. Among the particulate preparations, lysolecithinase activity of the intestine exceeded by far similar fractions of other organs. Among the soluble fractions, the 100,000xg supernatant of lung had the highest activity. Under the assay conditions used, most of the lysolecithinase activity of all organs was particulate. |
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