不同发育时期的鸡胚色素上皮对体外视网膜细胞生长的作用(简报)

眼正常发育中,色素上皮层和视刚膜之间在结构上存在着紧密的接触,功能上也相互依赖,但色素上皮的作用不甚清楚。很多脊椎动物的视网膜如果失去了与色素上皮层的紧密

EFFECT OF PIGMENTED EPITHELIUM FROM DIFFERENT DEVELOPMENTAL STAGES OF CHICK EMBRYO ON CELL GROWTH OF RETINAL EXPLANTS

The model system to investigate the effect of retinal pigmented epithelium (PE) on the retinal development in vitro has been established in this laboratory. Chick retina separated from 5-day-old embryo (E 5) were cut into strips and explanted on the collagen substratum either in close contact with retinal PE (RPE), or without PE (R). The rates of cell proliferation of retinal strips cultured for 48 hr were measured by the uptake of radioactive thymidine and DNA contents. Both paramaters in RPE were increased to values ranging from 137 to 167% when PE was taken from E 5 and E 6. However PE taken from E 7, E 8 and E 9 had no effect on cell proliferation (Table 1). The rate of cell proliferation of retina were increased both when seperated retina and PE of E 5 either from same or from an other eye closely contact again and when retina and PE of E 5 were explanted together without seperation. However the rates of cell proliferation were remained without much change when a millipore filter existed between retina and PE of E5 as well as the retina was inverted, the ganglion cell layer contacted with PE.The neurite outgrowth from retina explant with and without PE of E5 or E6 were also different. After culture for 24 hr the fiber length of neurite growing in RPE was only 36--39% of that in R. After 48 hr it was about 70% of that in R (Table 2).This results suggested that the developmental stage of PE and the direct cell-to-cell contact of PE from E 5 with photoreceptor layer of retina was important for the retinal cell proliferation. But PE had negative influence on neurite growth of retina in culture. (Projects Supported by the Science Fund of the Chinese Academy of Sciences)