Characterization of wheat o-diphenolase isoenzyme |
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Authors: | Francesco S Interesse Pacifico Ruggiero Gerolmina DAvella Francesco Lamparelli |
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Institution: | 1. Istituto di Chimica Agraria, Universitá degli Studi di Bari, Via Amendola 165/a, 70126, Bari, Italy |
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Abstract: | A highly purified isoenzyme of wheat o-diphenolase was characterized. The isoenzyme had a MW of ca 115 000, as determined by Sephadex G-100 gel filtration. The copper content was 0.20%, and the amino acid composition was determined. Two subunits (MWs ca 30 000 and 23 500) were detected by SDS gel electrophoresis. The Km was found to be 5.1 mM for 4-methylcatechol and kinetic analysis showed that the isoenzyme exhibited substrate inhibition. The isoenzyme was characterized by its response to some inhibitors. |
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Keywords: | Gramineae wheat isoenzyme MW copper content amino acid composition kinetic properties inhibitors |
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