Metabolism of [3H]gibberellin A4 in somatic suspension cell cultures of carrot |
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Authors: | Masaji Koshioka Alan Jones Mayumi N Koshioka Richard P Pharis |
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Institution: | 1. Division of Biochemistry, Department of Chemistry, University of Calgary, Calgary, Alberta, T2N 1N4, Canada |
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Abstract: | The native gibberellin A4 (GA4), in radioactive form (1,2-3H]GA4, 1.06 Ci/mmol), was fed to carrot somatic cell cultures (suspension and immobilized cell systems) and its metabolism over a 48 hr period was investigated. It was found that the 3H]GA4 was metabolized to at least two GAs, 3H]GA1 and 3H]GA8, six GA glucosyl conjugates, 3H]GA1-0(3)-glucoside, 3H]GA1-0(13)-glucoside, 3H]GA1-glucosyl ester, 3H]GA4-glucoside, 3H]GA4-glucosyl ester, a 3H]GA8 glucosyl conjugate(s) and a previously unknown 3H]GA1 glucosyl conjugate (3H]GA1-0(3,13)-diglucoside-like compound). The GA1-diglucoside-like compound was found only in extracts of cells and was present in significant amounts (33 % of total extractable radioactivity). All other metabolites were present in both cells and medium. For extracts of the medium, no differences between the suspension and immobilized cultures existed in types of 3H]GA4 metabolites although quantitative differences were apparent. |
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Keywords: | Umbelliferae somatic cell cultures metabolism GAs GA glucosyl conjugates |
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