Alkaloid production in cultured cells of Dioscoreophyllum cumminsii

Jatrorrhizine and magnoflorine were isolated from cultured cells of Dioscoreophyllum cumminsii. The jatrorrhizine content in cultured cells was 40–100 times higher than that of the intact plant, but columbamine, which is a minor component in the original plant, was not detected. Moreover, it was observed that the addition of 1AA or NAA to the growth medium increases the alkaloid content as compared with 2,4-D.