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The terminal structures of feather keratin mRNA
Authors:C. Phillip Morris  George E. Rogers
Affiliation:(1) Department of Biochemistry, University of Adelaide, 5001 Adelaide, South Australia, Australia
Abstract:Terminal labeling of embryonic feather keratin mRNA with [3H]KBH4 followed by digestion with ribonuclease T1 and T2, alkaline phosphatase, snake venom phosphodiesterase, and nucleotide pyrophosphatase and analysis of the products by high voltage paper electrophoresis, indicated the presence of the sequence m7G(5prime)ppp(5prime)N at the 5prime-end of the mRNA. Ribonuclease T1 and A digests of the terminally labeled, and also of unlabeled mRNA followed by fractionation on denaturing polyacrylamide gels indicated the presence of polyadenylate tracts ranging in size from 45 to 165 nucleotides at the 3prime-end of the mRNA.
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