A paracentric inversion suppresses genetic recombination at the <Emphasis Type="Italic">FON3</Emphasis> locus with breakpoints corresponding to sequence gaps on rice chromosome 11L |
| |
Authors: | Li Jiang Wenli Zhang Zhihui Xia Guanghuai Jiang Qian Qian Aili Li Zhukuan Cheng Lihuang Zhu Long Mao Wenxue Zhai |
| |
Institution: | (1) Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China;(2) Graduate University of Chinese Academy of Sciences, Beijing, 100039, China;(3) Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310006, China;(4) National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China;(5) Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115, USA |
| |
Abstract: | Paracentric inversion is known to inhibit genetic recombination between normal and inverted chromosomal segments in heterozygous
arrangements. Insect inversion polymorphisms have been studied to reveal adaptive processes for maintaining genetic variation.
We report the first paracentric inversion in rice (Oryza sativa), which was discovered in our effort to clone the floral organ number gene FON3. Recombination at the FON3 locus on the long arm of chromosome 11 was severely suppressed over a distance of more than 36 cM. An extensive screening
among 8,242 F2 progeny failed to detect any recombinants. Cytological analysis revealed a loop-like structure on pachytene chromosomes,
whereas FISH analysis showed the migration of a BAC clone from a distal location to a position closer to the centromere. Interestingly,
the locations where the genetic recombination suppression began were coincided with the positions of two physical gaps on
the chromosome 11, suggesting a correlation between the physical gaps, the inversion breakpoints. Transposons and retrotransposons,
and tandemly arranged members of gene families were among the sequences immediately flanking the gaps. Taken together, we
propose that the genetic suppression at the FON3 locus was caused by a paracentric inversion. The possible genetic mechanism causing such a spontaneous inversion was proposed. |
| |
Keywords: | Rice FON3 Genetic mapping Chromosomal inversion Recombination suppression |
本文献已被 PubMed SpringerLink 等数据库收录! |
|