The preparation of vesicles bounded by single phospholipid bilayers and loaded with acetylcholine

Summary Vesicles bounded by single lipid bilayer membranes (outside diameter 350 to 400 Å) can be made to enclose aqueous solutions of a variety of compounds. The procedure to prepare such vesicles in suspension is demonstrated with solutions of acetylcholine (ACh) in different concentrations as inside media. Several points are submitted as proof that the vesicles can contain a particular compound not present in the outside aqueous solution.The results of this work were presented in part at the First European Biophysics Congress, Baden near Vienna, Austria, September 14–17, 1971. Supported by a grant from the Medical Research Council of Canada.Scholar of the Medical Research Council of Canada.