A simple,versatile feeder layer system for Brassica oleracea protoplast culture |
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Authors: | Thomas W Walters Elizabeth D Earle |
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Institution: | (1) Department of Plant Breeding and Biometry, Cornell University, 14853-1902 Ithaca, NY, USA |
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Abstract: | Protoplasts from cauliflower (Brassica oleracea ssp. botrytis) and broccoli (ssp. italica) leaves and hypocotyls were successfully cultured on membrane filters over a feeder layer of cells from a B. campestris suspension culture. Cells from rice, tomato and tobacco suspensions were not as effective as the B. campestris cells. Plants were recovered from protoplasts of previously recalcitrant Brassica genotypes. Protoplasts cultured in low numbers (10–100) on the feeder layer divided and formed colonies capable of plant regeneration, as did fused protoplasts.Abbreviations BA
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- NAA
naphthaleneacetic acid
- PCV
packed cell volume |
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