Complete Genomic Structure of the Bloom-forming Toxic Cyanobacterium Microcystis aeruginosa NIES-843 |
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Authors: | Kaneko Takakazu; Nakajima Nobuyoshi; Okamoto Shinobu; Suzuki Iwane; Tanabe Yuuhiko; Tamaoki Masanori; Nakamura Yasukazu; Kasai Fumie; Watanabe Akiko; Kawashima Kumiko; Kishida Yoshie; Ono Akiko; Shimizu Yoshimi; Takahashi Chika; Minami Chiharu; Fujishiro Tsunakazu; Kohara Mitsuyo; Katoh Midori; Nakazaki Naomi; Nakayama Shinobu; Yamada Manabu; Tabata Satoshi; Watanabe Makoto M |
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Institution: | 1 Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan
2 National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
3 Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan |
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Abstract: | The nucleotide sequence of the complete genome of a cyanobacterium,Microcystis aeruginosa NIES-843, was determined. The genomeof M. aeruginosa is a single, circular chromosome of 5 842 795base pairs (bp) in length, with an average GC content of 42.3%.The chromosome comprises 6312 putative protein-encoding genes,two sets of rRNA genes, 42 tRNA genes representing 41 tRNA species,and genes for tmRNA, the B subunit of RNase P, SRP RNA, and6Sa RNA. Forty-five percent of the putative protein-encodingsequences showed sequence similarity to genes of known function,32% were similar to hypothetical genes, and the remaining 23%had no apparent similarity to reported genes. A total of 688kb of the genome, equivalent to 11.8% of the entire genome,were composed of both insertion sequences and miniature inverted-repeattransposable elements. This is indicative of a plasticity ofthe M. aeruginosa genome, through a mechanism that involveshomologous recombination mediated by repetitive DNA elements.In addition to known gene clusters related to the synthesisof microcystin and cyanopeptolin, novel gene clusters that maybe involved in the synthesis and modification of toxic smallpolypeptides were identified. Compared with other cyanobacteria,a relatively small number of genes for two component systemsand a large number of genes for restriction-modification systemswere notable characteristics of the M. aeruginosa genome. |
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Keywords: | cyanobacterium M aeruginosa microcystin water bloom genome sequence |
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