Life cycle and morphology of Physarum pusillum (Myxomycetes) on agar culture |
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Authors: | Xiaoshu Chen Shuo Gu He Zhu Zhuang Li Qi Wang Yu Li |
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Institution: | 1. Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, PR China;2. College of Plant Protection, Shandong Agricultural University, 271018, PR China |
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Abstract: | Myxomycetes (slime molds) are unique eukaryotic microorganisms with both characteristics of fungi and amoebae. Artificial cultures grown under controlled conditions were used to study the life cycle and morphogenesis. Physarum pusillum was collected from the field. Spores were inoculated and cultured with the hanging drop method. The complete life cycle was observed from spore to spore on agar without adding any solid nutrients or bacteria as food. Life cycle morphological characteristics were described for spore germination, myxamoebae, zygote, plasmodium and sporangia formation. |
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