BRCA1相关蛋白1对肾癌增殖和侵袭的实验研究

目的:研究BRCA1相关蛋白(BRCA1-associated protein-1,BAP1)对肾癌细胞增殖和侵袭的影响。方法:通过含有不同BAP1和HAT1载体的慢病毒感染后用嘌呤霉素筛选的方法在肾癌细胞系中构建BAP1稳定低表达、HAT1稳定高表达、HAT1稳定低表达以及BAP1低表达且HAT1低表达的慢病毒细胞稳转株。通过WB和PCR验证BAP1在肾癌细胞系中对于HAT1的调控。使用CCK-8细胞增殖和TRANSWELL侵袭实验检测对于肾癌细胞增殖和侵袭能力的影响。利用免疫组化和临床回访数据分析其临床意义。结果:肾癌细胞系中BAP1表达降低后HAT1表达升高。BAP1低表达部分通过了HAT1表达升高促进肾癌的增殖和侵袭能力。在肾癌组织中BAP1的表达与HAT1表达具有相关性(P0.05)。结论:BAP1敲低的肾癌细胞中HAT1特异性的高表达可能是导致肾癌细胞增殖和侵袭能力增强的原因。BAP1与HAT1在肾癌组织中的表达具有显著相关性。

Experimental Study of BRCA1-related Protein 1 on Proliferation and Invasion of Renal Cell Carcinoma

ABSTRACT Objective: To investigate the effects of BRCA1-associated protein-1 (BAP1) on proliferation and invasion of renal cell carcinoma cells. Methods: The lentivirus containing BAP1 and HAT1 vectors was infected and screened with puromycin. The BAP1 stably expressed low in the renal cancer cell line, the HAT1 stably expressed high and low, and the BAP1 low expression and the HAT1 low expression of the lentiviral cells were stable Transfer strain. The regulation of HAT1 by BAP1 in renal cancer cell lines was verified by WB and PCR. The effect on proliferation and invasion of renal cancer cells was examined using CCK-8 cell proliferation and TRANSWELL invasion assays. The clinical significance was analyzed by immunohistochemistry and clinical return visit data. Results: HAT1 expression is increased after decreased expression of BAP1 in renal cancer cell lines. The low expression of BAP1 partially promotes the proliferation and invasion of renal cancer by increasing the expression of HAT1. BAP1 expression is correlated with HAT1 expression in renal cell carcinoma(P<0.05). Conclusion: High expression of HAT1 specificity in BAP1 knockdown renal cancer cells may be responsible for the enhancement of renal cancer cell proliferation and invasion. There was a significant correlation between the expression of BAP1 and HAT1 in renal cell carcinoma.