Transformation of the mushroom species Hypsizigus marmoreus,Flammulina velutipes,and Grifola frondosa by an Agrobacterium-mediated method using a universal transformation plasmid |
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| Authors: | Kanako Hatoh Kosuke Izumitsu Atsushi Morita Kiminori Shimizu Akira Ohta Masataka Kawai Takashi Yamanaka Hitoshi Neda Yuko Ota Chihiro Tanaka |
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| Institution: | 1. Laboratory of Environmental Mycoscience, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan;2. Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan;3. Nara Forest Research Institute, Takatori, Nara Pref. 635-0133, Japan;4. Shiga Forest Research Centre, Yasu, Shiga Pref. 520-2321, Japan;5. Forestry and Forest Products Research Institute, Tsukuba, Ibaraki 305-8687, Japan |
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| Abstract: | Agrobacterium tumefaciens-mediated transformation (AMT) was successfully applied to mycelia of the 3 economically important mushrooms Hypsizigus marmoreus, Flammulina velutipes, and Grifola frondosa. We used the hygromycin B resistance gene (hph) under the control of the Cryptococcus neoformans actin promoter. Eighty-six resistant strains of H. marmoreus, 4 of F. velutipes, and 2 of G. frondosa were obtained. All transformants were highly resistant to hygromycin B, suggesting that the C. neoformans actin promoter has a potential universal promoter activity in basidiomycetes. Southern analysis revealed random but single integration of the hph gene. |
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