高糖环境下持续性牵张力对子宫平滑肌细胞白介素-1和白介素-6表达的影响

目的:探讨高糖环境下持续性牵张力对大鼠子宫平滑肌细胞白介素-1(IL-1)、白介素-6(IL-6)表达的影响。方法:体外培养大鼠子宫平滑肌细胞,高糖作用不同时间后,观察高糖状态下大鼠子宫平滑肌细胞IL-1、IL-6表达变化情况。对高糖状态下的肌细胞施加持续性牵张力,明确牵张力对肌细胞IL-1、IL-6表达的影响以及高糖和牵张力之间的协同作用,同时采用晚期糖基化终末产物(AGEs)抑制剂拮抗高糖作用作为参照,并对结果进行分析。结果:随着高糖作用时间增加,肌细胞IL-1、IL-6表达也随之升高。牵张力也可促进肌细胞IL-1、IL-6表达增加,并可与高糖状态产生协同作用,这一过程可被高糖抑制剂部分阻断,但不能完全阻断。结论:高糖状态及牵张力均可促进肌细胞IL-1、IL-6表达增加,并有一定协同作用,AGEs参与了这一过程,但并不是唯一途径。

Effect of Continuous Stretch on Inflammatory Factor Expression of Uterine Smooth Muscle Under High Glucose Circumstance

ABSTRACT Objective: To analyze the effect of continuous stretch on Interleukin -1(IL-1) and Interleukin -6(IL-6) expression of uterine smooth muscle cells under high glucose circumstance. Methods: Rat uterine smooth muscle cells were separated and cultured under high glucose circumstance for different durations, then we detected IL-1 and IL-6 expression changes of smooth muscle cells. Continuous stretch was forced on cells under high glucose circumstance to determine synergistic effect of stretch and high glucose. At the same time, the antagonistic effect of advanced glycation end products (AGEs) inhibitors on hyperglycemia was used as a reference, and the results were analyzed. Results: With the increase of duration under high glucose circumstance, IL-1, IL-6 expression of muscle cell also increased. Continuous stretch could also promote IL-1, IL-6 expressions and there exist a synergistic effect between stretch and high glucose. This process could partly be blocked by high glucose inhibitor,but not completely. Conclusion: Both continuous stretch and high glucose could promote IL-1, IL-6 expressions and there exist a synergistic effect between them. AGEs was involved in this process, but this is not the only signal pathway.