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High mycorrhizal specificity in the mycoheterotrophic Burmannia nepalensis and B. itoana (Burmanniaceae)
Authors:Yuki Ogura-Tsujita  Hidetaka Umata  Tomohisa Yukawa
Affiliation:1. Botanical Gardens, Tohoku University, 12-2 Kawauchi, Aoba-ku, Sendai, Miyagi 980-0862, Japan;2. 5211 Kita-Takanabe, Takanabe-cho, Koyu-gun, Miyazaki 885-0002, Japan;3. National Museum of Nature and Science, 4-1-1 Amakubo, Tsukuba, Ibaraki 305-0005, Japan
Abstract:Mycorrhizal fungi of mycoheterotrophic Burmannia nepalensis and B. itoana were identified by molecular identification methods based on fungal SSU nrDNA region. In B. nepalensis, RFLP patterns and sequences from all root samples from 14 individuals were identical. A single fungal sequence was also obtained from B. itoana roots from three individuals. Phylogenetic analysis showed that the fungal sequences from these two species are included in Glomeraceae (former Glomus group A). Our results indicate that the two Burmannia species are associated with narrow phylogenetic ranges of arbuscular mycorrhizal fungi.
Keywords:Arbuscular mycorrhiza  Glomeraceae  Mycoheterotroph
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