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Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase
Authors:S Tajima  R Hayashi  S Tsuchiya  Y Miyake  A Yamamoto
Affiliation:1. Department of Etiology and Pathophysiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565, Japan;2. Department of Pediatrics, Tohoku University School of Medicine, Sendai 980, Japan;1. Graduate School of Science and Engineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan;2. Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate, Suita, Osaka 564-8680, Japan;1. Department of Pediatrics, Kobe University Graduate School of Medicine, Hyogo, Japan;2. Department of Neurology, Hyogo Prefectural Kobe Children’s Hospital, Hyogo, Japan;3. Department of Pediatric Critical Care Medicine, Hyogo Prefectural Kobe Children’s Hospital, Hyogo, Japan;4. Department of Brain Development and Neural Regeneration, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo, Japan;5. Department of Emergency and General Pediatrics, Hyogo Prefectural Kobe Children’s Hospital, Hyogo, Japan;1. Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD, USA;2. Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, MD, USA;3. Department of Pathology, Walter Reed National Military Medical Center, Bethesda, MD, USA;4. Department of Microbiology, Icahn School of Medicine, New York, NY, USA;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China;3. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China
Abstract:A human cell line established from a patient of an acute monocytic leukemia (THP-1) retained an ability to synthesize and secrete plasma apolipoprotein E like protein. The protein was identified with monospecific antibody raised against human plasma apolipoprotein E. The cells also secreted lipoprotein lipase (EC 3.1.1.34). The enzyme was characterized as lipoprotein lipase on the basis of the requirement of apolipoprotein C-II as an activator and the inhibition of its activity by sodium chloride. The secretion of both apolipoprotein E and lipoprotein lipase was markedly enhanced in the process of differentiation into macrophage-like cells by the addition of 4 beta-phorbol 12 beta-myristate 13 alpha-acetate.
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